Manny Pacquiao has called for punishment and a rematch after Floyd Mayweather was accused of violating anti-doping rules in the buildup to their mega-fight in May.

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Pacquiao, who lost a unanimous decision to Mayweather in Las Vegas, urged Nevada sports officials to “impose the appropriate sanction” on the unbeaten American.

Mayweather and the United States Anti-Doping Agency (USADA) have both insisted the boxer’s actions were legal after it emerged he was injected with vitamins and minerals before the fight.

But Pacquiao has questioned why USADA only informed the Nevada State Athletic Commission (NSAC) about the infusion three weeks after the bout, by which time Mayweather already had a USADA exemption.

“Are they hiding something? For the sake of fairness and for the good of the sport, NSAC must be consistent,” he said.

A report on the SB Nation sports news website said Mayweather had broken World Anti-Doping Agency (WADA) rules by having an IV infusion at his home in Las Vegas on May 1, the day before the fight.

WADA say IV infusions are prohibited because they can be used to mask performance-enhancing drugs, increase plasma volume levels and distort the values of an athlete’s biological passport. But a USADA source said that because Mayweather obtained a therapeutic use exemption, no offence had been committed.

Pacquiao said he was treated unfairly because the Nevada body refused him a USADA-approved painkiller for the fight, when he was carrying a shoulder injury.

“That is why I want a rematch. One without any injury, with fair play. No favouritism,” added the Filipino