Nine-time world champion Rossi was within touching distance of a 10th title on his Yamaha last season until an ill-judged kick at Honda rival Marquez at the penultimate race in Malaysia wrecked his chances.

He was punished by being forced to start the title-decider in Valencia at the back of the grid, a penalty which allowed his Yamaha teammate Jorge Lorenzo to take the championship by just five points.

Rossi finished fourth but accused Marquez of deliberately helping fellow Spaniard Lorenzo to the finish line at his expense by acting effectively as a bodyguard.

“I allowed myself to be tricked by Marquez. He really betrayed me. He said he was a fan but it was all lies, bull****,” said Rossi on the eve of his 21st season as a professional rider.

The Italian added: “It would be hypocritical to say that it’s over and shake his hand, because it’s not true. Our relationship can never be recovered. I like to do real things, not to pretend.”

Rossi and Lorenzo are teammates again but they are far from friends, a factor which adds to Yamaha’s conundrum at the end of the year when both men will be out of contract.

Yamaha Tech 3 boss Herve Poncharal said: “What happened (last year) took its toll, so whatever happens, it seems tricky to consider keeping Lorenzo and Rossi at Yamaha in 2017. If Lorenzo stays, I don’t know if Rossi will want to stay, and vice versa.”

The public disputes between Rossi, triple MotoGP champion Lorenzo and Marquez, a two-time winner of the elite title, have also attracted the attention of legendary 15-time champion Giacomo Agostini.

Talking about Rossi and Marquez, he told motogp.com: “I would like to see a bit more respect between them because for me this is the most important thing. I don’t think that true friendship can exist between rivals in our sport, but the important thing is to have respect for each other as that leads to great racing and this is what I hope we will see.”

Marquez, the 2013 and 2014 champion again sees Lorenzo, Rossi and Honda teammate Dani Pedrosa as being the major title rivals.

He said: “The strength of Lorenzo is his speed; the guy is fast and once he’s far ahead on track it’s very hard to beat him. Valentino has a lot of experience and Dani’s comprehensive understanding of a situation is his strong point. But you never know who else could surprise you, especially seeing how the Ducati team are getting stronger with a lot of technical improvements.”

The 2016 championship starts under the lights in Qatar and ends in Valencia in November with 21 riders.